Expressions of COX-2, PKC-α and miR-101 in gastric cancer and their correlations / 南方医科大学学报
Journal of Southern Medical University
; (12): 559-562, 2013.
Article
in Zh
| WPRIM
| ID: wpr-306513
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expressions of miR-101, protein kinase C-α (PKC-α), and cyclooxygenase-2 (COX-2) in gastric cancer (GC) tissue and their correlations.</p><p><b>METHODS</b>RT-qPCR was used to examine miR-101 expression and Western blotting employed to detect PKC-α and COX-2 expressions in 57 cases of gastric cancer tissues and paired normal gastric mucosal tissues.</p><p><b>RESULTS</b>The gastric cancer tissues showed a significantly lower miR-101 expression (Z=6.102, P<0.05) but significantly higher expressions of COX-2 (Z=14.436, P<0.05) and PKC-α (Z=6.955, P<0.05) than the normal gastric tissues. The expression of COX-2 protein was significantly correlated with the degree of differentiation, invasion depth, lymph node metastasis and TNM stage (P<0.05); PKC-α protein expression was associated with lymph node metastasis and TNM stage (P<0.05). PKC-α expression was positively correlated (r=0.531, P<0.05) and miR-101 expression negatively correlated (r=-0.627, P<0.05) with COX-2 expression in gastric cancer tissues.</p><p><b>CONCLUSIONS</b>miR-101, PKC-α and COX-2 all play a role in the tumorigenesis and progression of gastric cancer. miR-101 and PKC-α might be new potential therapeutic targets for inhibiting COX-2 in gastric cancer.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
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Stomach Neoplasms
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MicroRNAs
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Cyclooxygenase 2
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Protein Kinase C-alpha
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Gastric Mucosa
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Metabolism
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Neoplasm Staging
Limits:
Adult
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Aged
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Aged80
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Female
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Humans
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Male
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2013
Type:
Article