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The expression of X-linked inhibitor of apoptosis protein and cell apoptosis in caerulein-stimulated rat pancreatic acinus AR42J cell lines / 生物医学工程学杂志
Journal of Biomedical Engineering ; (6): 332-351, 2011.
Article in Chinese | WPRIM | ID: wpr-306564
ABSTRACT
To study the expression of X-linked inhibitor of apoptosis protein (XIAP) and cell apoptosis in vitro model of acute pancreatitis (AP), we carried out experiments to stimulate AR42J cell line with caerulein (10(-8) mol/L) for 12 hours, then collected cells at various time points (0 h, 4 h, 8 h, 12 h, and 24 h, respectively). We then observed the morphologic changes of AR42J cells with the stimulation of caerulein with electronic microscope. The gene expression of XIAP, caspase-3 and caspase-9 was detected using real-time fluorescence quantitative polymerase chain reaction (FQ-PCR), and the protein expression of XIAP was assessed by western blot. The activation of nuclear factor-kappa B (NF-kappaB) was measured by flow cytometry (FCM). With the stimulation of caerulein, the expression of XIAP and the NF-kappaB activation could first decrease and then increase, but the change of caspase-3 and caspase-9 expressions were opposite. XIAP may inhibit the cell apoptosis in rat pancreatic acinus AR42J cell lines at first with the stimulation of caerulein, then NF-kappaB can upgrade the expression of XIAP and increase the cell apoptosis.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pancreas / Pancreatitis / Pharmacology / Physiology / Ceruletide / Cell Line / NF-kappa B / Apoptosis / Cell Biology / X-Linked Inhibitor of Apoptosis Protein Type of study: Prognostic study Limits: Animals Language: Chinese Journal: Journal of Biomedical Engineering Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pancreas / Pancreatitis / Pharmacology / Physiology / Ceruletide / Cell Line / NF-kappa B / Apoptosis / Cell Biology / X-Linked Inhibitor of Apoptosis Protein Type of study: Prognostic study Limits: Animals Language: Chinese Journal: Journal of Biomedical Engineering Year: 2011 Type: Article