Enhancement of cytotoxicity of cantionic antimicrobial peptide in tumor cells by conjugation to cell-penetrating peptide / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 110-114, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-306611
ABSTRACT
Due to their lower risk for induction of resistance, membrane-active antimicrobial peptides with anticancer effect are attractive in cancer therapy. Because cell binding contributes to the cytotoxicity of peptide, it is possible to enhance the cytotoxicity of antimicrobial peptide in tumor cells by conjugation to a cell-penetrating peptide (CPP). In this paper, a fusion peptide MPGA by conjugation of antimicrobial peptide MP to CPP Antp at its N-terminus was constructed. After compared the cytotoxicity of unconjugated MP with that of the fusion peptide, it was found that MPGA showed higher cytotoxicity than that of unconjugated MP. And the fusion peptide MPGA induced cell death in tumor cells by membrane disruption. These results demonstrated that the cytotoxicity of antimicrobial peptide can be significantly enhanced by conjugation to CPP, which might be an effective way to develop novel anticancer drugs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Pharmacokinetics
/
Cell Membrane Permeability
/
Antimicrobial Cationic Peptides
/
Cell Line, Tumor
/
Antineoplastic Agents
Limits:
Humans
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2011
Type:
Article
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