Curcumine inhibits migration and invasion of hepatic stellate cells by reducing MMP-2 expression and activity / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 835-838, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-306633
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the molecular mechanism of the inhibitory effect of curcumine on the migration and invasion of hepatic stellate cells (HSC).</p><p><b>METHODS</b>Rat hepatic stellate cells were cultured and activated with ConA. Matrix metalloproteinase-2 (MMP-2) expression and activity was determined by Western blot and gelatin zymography. Migration and invasion of HSC was assessed by wound healing assay and modified Boyden chamber assay.</p><p><b>RESULTS</b>Curcumine reduced the level and activity of MMP-2 expression in activated HSC in a dose-dependent manner. When treated with 25, 50 or 100 micromol/L curcumine, the expression of MMP-2 was reduced by 21.8%+/-5.1%, 65.5%+/-9.2% or 87.9%+/-11.5% (P < 0.05), and the activity of MMP-2 was also significantly reduced by curcumine. Migration and invasion of activated HSC was also inhibited by curcumine in a dose-dependent way. When treated with 25, 50 or 100 micromol/L curcumine, the migration of activated HSC was reduced by 27.5%+/-5.8%, 54.4%+/-7.6% or 67.1%+/-9.3% (P < 0.05), and the invasion of activated HSC was also significantly reduced by curcumine.</p><p><b>CONCLUSION</b>Curcumine inhibits migration and invasion of activated HSC by reducing MMP-2 expression and activity.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Cell Movement
/
Cells, Cultured
/
Blotting, Western
/
Concanavalin A
/
Tissue Inhibitor of Metalloproteinase-2
/
Matrix Metalloproteinase 2
/
Curcumin
/
Cell Biology
/
Dose-Response Relationship, Drug
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2009
Type:
Article
Similar
MEDLINE
...
LILACS
LIS