Alteration of Expression of Ca(2+) Signaling Proteins and Adaptation of Ca(2+) Signaling in SERCA2(+/-) Mouse Parotid Acini
Yonsei Medical Journal
;
: 311-321, 2008.
Article
in English
| WPRIM
| ID: wpr-30669
ABSTRACT
PURPOSE:
The sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA), encoded by ATP2A2, is an essential component for G-protein coupled receptor (GPCR)-dependent Ca(2+) signaling. However, whether the changes in Ca(2+) signaling and Ca(2+) signaling proteins in parotid acinar cells are affected by a partial loss of SERCA2 are not known. MATERIALS ANDMETHODS:
In SERCA2(+/-) mouse parotid gland acinar cells, Ca(2+) signaling, expression levels of Ca(2+) signaling proteins, and amylase secretion were investigated.RESULTS:
SERCA2(+/-) mice showed decreased SERCA2 expression and an upregulation of the plasma membrane Ca(2+) ATPase. A partial loss of SERCA2 changed the expression level of 1, 4, 5-tris-inositolphosphate receptors (IP(3)Rs), but the localization and activities of IP3Rs were not altered. In SERCA2(+/-) mice, muscarinic stimulation resulted in greater amylase release, and the expression of synaptotagmin was increased compared to wild type mice.CONCLUSION:
These results suggest that a partial loss of SERCA2 affects the expression and activity of Ca(2+) signaling proteins in the parotid gland acini, however, overall Ca(2+) signaling is unchanged.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Parotid Gland
/
Carbachol
/
Immunohistochemistry
/
Signal Transduction
/
Blotting, Western
/
Calcium
/
Mice, Knockout
/
Calcium Signaling
/
Sarcoplasmic Reticulum Calcium-Transporting ATPases
/
Inositol 1,4,5-Trisphosphate Receptors
Limits:
Animals
Language:
English
Journal:
Yonsei Medical Journal
Year:
2008
Type:
Article
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