Study on sero-pharmacology of fresh Gecko Swinhonis Gunther freeze-dried powder in inducing cell apoptosis of C6 glioma cells in mice / 中国中西医结合杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore the effect of Gecko Swinhonis Gunther freeze-dried powder (GFP) in inducing apoptosis of C6 glioma cells.</p><p><b>METHODS</b>Thirty mice were randomly divided into three groups and treated with intraperitoneally injection of cisplatin, orally taken GFP or distilled water respectively. After treatment for 20 days, the serum of mice was collected for treating the C6 glioma cells. The apoptosis state of cells was observed by morphological examination, flow cytometry and TUNEL method, and the cell apoptosis related gene expression of bcl-2 and bax were determined by S-P immunocytochemical assay.</p><p><b>RESULTS</b>The C6 glioma cell apoptosis induction of GFP contained serum in vitro could be confirmed by morphological examination, flow cytometry analysis and TUNEL method. Comparison between the GFP treated group and the blank control group on intracellular bcl-2 gene expression showed no difference, but bax gene expression was higher in the GFP treated group.</p><p><b>CONCLUSION</b>GFP contained serum could induce C6 glioma cell apoptosis, its mechanism might be related with the up-regulation of bax gene.</p>