Antagonistic effect of yishen ruanjian san contained serum against aristolochic acid in antagonizing human renal interstitial fibroblasts / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 811-815, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-306777
ABSTRACT
<p><b>OBJECTIVE</b>To study whether yishen ruanjian san contained serum (S-YRS) could intervene the action of aristolochic acid (AA) in antagonizing human renal interstitial fibroblasts (hRIFs) to induce extracellular matrix (ECM) accumulation.</p><p><b>METHODS</b>AA-Na 40 microg/ml, with or without 10% S-YRS, was co-cultured with hRIFs, then the hRIFs mRNA of transforming growth factor-beta1 (TGF-beta1), connective tissue growth factor (CTGF), plasminogen activator inhibitor-1 (PAI-1), tissue inhibitor of metalloproteinase-1 (TIMP-1) and type I collagen (Col I) in the cultured cells were detected by RT-PCR, and their protein expression monitored with ELISA and Western blot respectively.</p><p><b>RESULTS</b>The mRNA and protein expression of all the above-mentioned factors were significantly up-regulated by AA-Na (P < 0.05). Excepting PAI-1, the enhanced mRNA and protein expression were significantly down-regulated by S-YRS (P < 0.05).</p><p><b>CONCLUSION</b>S-YRS could down-regulate the hRIF to promote the expression of ECM synthesis factors and inhibit the ECM degradation factors in hRIFs, so as to antagonize the AA stimulated accumulation of ECM such as Col I.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
RNA, Messenger
/
Drugs, Chinese Herbal
/
Pharmacokinetics
/
Cells, Cultured
/
Transforming Growth Factor beta
/
Rats, Sprague-Dawley
/
Tissue Inhibitor of Metalloproteinase-1
/
Aristolochic Acids
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2004
Type:
Article
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