Local proinflammatory effects of repeated skin exposure to warfarin, an anticoagulant rodenticide in rats / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 180-189, 2011.
Article
in En
| WPRIM
| ID: wpr-306874
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effects of epicutaneous application of anticoagulant warfarin, by examining the presence of tissue injury and immune/inflammatory activity in exposed skin.</p><p><b>METHODS</b>Rats were exposed to warfarin by applying 10 μg of warfarin-sodium to 10-12 cm(2) skin (range 0.8-1 μg per 1 cm(2)) for 3 consecutive days. Tissue injury was evaluated by lipid peroxidation, histomorphological changes and signs of reparative activity in skin. T cell infiltration and selected aspects of epidermal cell activity were examined as indicators of immune/inflammatory skin response to warfarin application.</p><p><b>RESULTS</b>Repeated warfarin application exerted no effect on skin metabolic viability, but resulted in tissue injury (increased malondialdehyde, MDA, production, evident histo-morphological changes in epidermis and dermis depicting cell injury and death). Increased numbers of proliferating cell nuclear antigen (PCNA(+)) cells indicated reparative processes in injured skin. Infiltration of CD3(+) cells (T lymphocytes) along with the increased production of tumor necrosis factor-a (TNF-a) by epidermal cells from warfarin-treated skin and their co-stimulatory effect in an in vitro T-cell activation assay demonstrated immunomodulatory effects of epicutaneous warfarin.</p><p><b>CONCLUSION</b>Presented data have documented tissue damage associated with immune/inflammatory activity in skin exposed to warfarin. Observed effects are relevant to immunotoxic potential of this anticoagulant in settings of external exposure.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
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Pharmacology
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Physiology
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Rodenticides
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Skin
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Warfarin
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T-Lymphocytes
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Lipid Peroxidation
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Gene Expression Regulation
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CD3 Complex
Limits:
Animals
Language:
En
Journal:
Biomedical and Environmental Sciences
Year:
2011
Type:
Article