Partly purification of urinastatin by using affinity chromatography
Journal of Medical Research
;
: 34-39, 1998.
Article
in Vietnamese
| WPRIM
| ID: wpr-3071
ABSTRACT
A human urinary trypsin inhibitor (UTI) was purified by 80% amonium sulfat precipitation, affnity chromatography using trypsin cellulofine gel. Specificity inhibitory activity of solution after affinity chromatography was increase from 0.8 to 29.1 and its recovery was 20.8%. The reslt indicated that binding of trypsin to formyl- cellulofine and the affinity chromatography with trypsin cellulofine gel was successful. Capalary electrophoresis of urine, solutions before and after affinity chromatography also supported the above result.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Glycoproteins
/
Chromatography, Affinity
Language:
Vietnamese
Journal:
Journal of Medical Research
Year:
1998
Type:
Article
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