Remote postconditioning by brief renal ischemia and reperfusion reduces acute myocardial ischemia and reperfusion induced myocardial apoptosis in rabbits / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 757-760, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-307206
ABSTRACT
<p><b>OBJECTIVES</b>To observe the effects of renal ischemic postconditioning (RI-Post) on myocardial apoptosis in rabbits with acute myocardial ischemia and reperfusion.</p><p><b>METHODS</b>All rabbits were subjected to 60 minutes ischemia by left anterior descending coronary artery occlusion (LADO) and 6 hours reperfusion. The rabbits are randomly divided into 3 groups (n = 8 in each group) (1) Ischemia-reperfusion (IR) LADO and reperfusion without additional intervention; (2) RI-Post after 60 minutes of LADO, the left renal artery was occluded for 30 seconds and reperfused for 30 seconds and repeated 3 times, then the coronary artery was reperfused for 6 hours; (3) Medication intervention (MI) 10 minutes before coronary reperfusion, rabbits were treated with PKC antagonist GF109203X (0.05 mg/kg, IV), followed by RI-Post treatment and 6 hours coronary reperfusion. Myocardial apoptosis was measured by TUNEL and the myocardial Bcl-2 and Bax protein expressions were assessed by immunohistochemistry.</p><p><b>RESULTS</b>Compared with the IR group and the MI group, myocardial apoptosis was significantly reduced (P < 0.05) and the Bcl-2 protein expression increased (P < 0.01) while the Bax protein expression decreased (P < 0.05) in the RI-Post group.</p><p><b>CONCLUSIONS</b>Remote renal postconditioning applied right before the onset of coronary artery reperfusion can reduce the myocardial apoptosis induced by myocardial ischemia and reperfusion and up-regulate Bcl-2 while down-regulate Bax expression possibly by activation of protein kinase C.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Myocardial Reperfusion Injury
/
Apoptosis
/
Proto-Oncogene Proteins c-bcl-2
/
Ischemic Preconditioning
/
Bcl-2-Associated X Protein
/
Ischemia
/
Kidney Diseases
/
Metabolism
/
Myocardium
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Cardiology
Year:
2007
Type:
Article
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