Effects of Tiam1 on invasion and metastasis of breast carcinoma and its mechanisms / 中华肿瘤杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the significance of Tiam1 in invasion and metastasis of breast carcinoma and its mechanisms.</p><p><b>METHODS</b>Immunohistochemistry was used to detect Tiam1 expression in tumor tissue of 126 breast carcinomas. Tiam1 was silenced by siRNA in breast carcinoma cell line MDA-MB-435, then the expressions of phosphor-ERK 1, ERK 2 and VEGF were detected, and electrophoretic mobility shift assay (EMSA) was used to examine the transcription activiy of AP-1.</p><p><b>RESULTS</b>There was a significant relationship between Tiam1 expression and lymph node metastasis (P < 0.05). Furthermore, after silencing of Tiam1, the expressions of phosphor-ERK 1, ERK 2 and VEGF were decreased, and the transcription activity of AP-1 was down-regulated in the MDA-MB-435 cells.</p><p><b>CONCLUSION</b>Tiam1 is closely related with invasion and metastasis of breast carcinoma, and the cascade Tiam1 through ERK, AP-1 and VEGF pathways may play an important role in enhancing angiogenesis, therefore, to promote invasion and metastasis of breast carcinoma.</p>