Silencing effect of cell-specific RNA interference plasmid pPSMAe/p-shNS-ploy(A) loaded by transgenic vector Tf-PEG-PEI targeting nucleostemin on prostate cancer cells in vitro / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 725-729, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-307306
ABSTRACT
<p><b>OBJECTIVE</b>To explore the transgenic efficiency of non-viral vector Tf-PEG-PEI and the cell specific silencing effect of plasmid pPSMAe/p-shNS-ploy(A) on prostate cancer cells.</p><p><b>METHODS</b>Polyethyleneimine (PEI) was modified by using polyethylene glycol and transferrin to synthesize the non-viral vector Tf-PEG-PEI. NS-specific plasmids pPSMAe/p-shNS-ploy(A) and Tf-PEG-PEI were used to transfect prostate cancer LNCap and PC-3 cells. The changes of cell morphology, proliferation ability and cell cycle were studied after down-regulating the NS gene level.</p><p><b>RESULTS</b>Tf-PEG-PEI was successfully modified. After transfection, the PSMA-expressing LNCaP cells became larger and showed more pseudopodia, having a tendency to differentiate. Their cell proliferation ability was reduced, and the detection of cell cycle showed a decrease of S phase and an increase of G(1) phase after knocking down NS gene. These targets were not changed in non-PSMA-expresing PC-3 cells.</p><p><b>CONCLUSIONS</b>The non-viral vector Tf-PEG-PEI has a high ability to transfer targeted gene into target cells. The cellular specificity of short-hairpin RNA transcription driven by PSMAe/p is confirmed by silencing NS gene. The use of cell specific promoter may be an effective strategy of gene therapy for prostate cancer.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Plasmids
/
Polyethylene Glycols
/
Polyethyleneimine
/
Prostatic Neoplasms
/
RNA, Messenger
/
Transferrin
/
Nuclear Proteins
/
Transfection
/
Cell Cycle
Limits:
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2012
Type:
Article
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