Extraction and content determination of polysaccharides in Viscum coloratum / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a method for extraction and content determination of polysaccharide in Viscum coloratum.</p><p><b>METHOD</b>Polysaccharide was extracted by hot water, separated by ultrafiltration and ion-exchange chromatography. The content determination was performed at wavelength 490 nm with phenol-sulfuric acid as a chtomo-genic agent.</p><p><b>RESULT</b>The content of polyaccharide in V. coloratum, CVPS-III, and CVPS-III-C were respectively 4.93% (RSD 1.04%, n = 3), 43.28% (RSD 1.39%, n =3), 69.55% (RSD 1.62%, n = 3), and the average recovery was 96.07% (RSD 2.54%, n = 5).</p><p><b>CONCLUSION</b>The method was simple, rapid, and accurate.</p>