Extraction and content determination of polysaccharides in Viscum coloratum / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2387-2390, 2007.
Article
in Zh
| WPRIM
| ID: wpr-307514
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for extraction and content determination of polysaccharide in Viscum coloratum.</p><p><b>METHOD</b>Polysaccharide was extracted by hot water, separated by ultrafiltration and ion-exchange chromatography. The content determination was performed at wavelength 490 nm with phenol-sulfuric acid as a chtomo-genic agent.</p><p><b>RESULT</b>The content of polyaccharide in V. coloratum, CVPS-III, and CVPS-III-C were respectively 4.93% (RSD 1.04%, n = 3), 43.28% (RSD 1.39%, n =3), 69.55% (RSD 1.62%, n = 3), and the average recovery was 96.07% (RSD 2.54%, n = 5).</p><p><b>CONCLUSION</b>The method was simple, rapid, and accurate.</p>
Full text:
1
Index:
WPRIM
Main subject:
Plants, Medicinal
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Polysaccharides
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Chemistry
/
Reproducibility of Results
/
Chromatography, Ion Exchange
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Plant Stems
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Viscum
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Methods
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2007
Type:
Article