Simultaneous determination of four flavonoids in Malus prunifolia from Shanxi province by RP-HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2217-2219, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-307615
ABSTRACT
<p><b>OBJECTIVE</b>To develop an HPLC method for simultaneous determination of rutin, hyperoside, quercetin and kaempferol in Malus prunifolia from Shanxi province in China.</p><p><b>METHOD</b>The separation was performed on a Hypersil C18 column (4.6 mm x 250 mm, 5 microm), using a gradient elution with methanol-water containing 0.2% phosphoric acid as the mobile phase. The flow rate was 1.0 mL x min(-1), the detection wavelength was 360 nm and the temperature of column was 25 degrees T.</p><p><b>RESULT</b>The linear ranges of rutin, hyperoside, quercetin and kaempferol were 1.87-46.67 mg x L(-1), 6.40-160.0 mg x L(-1), 3.33-83.33 mg x L(-1), 0.80-20.00 mg x L(-1), respectively. The average recoveries (n=6) of the four constituents were 99.2% (RSD 2.9%), 98.2% (RSD 1.9%), 97.4% (RSD 2.3%), 97.2% (RSD 1.3%), respectively.</p><p><b>CONCLUSION</b>The method was simple, accurate and can be used to evaluate medicinal value of Malus prunifolia.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Flavonoids
/
Drugs, Chinese Herbal
/
Chemistry
/
China
/
Chromatography, High Pressure Liquid
/
Malus
/
Chromatography, Reverse-Phase
/
Methods
Country/Region as subject:
Asia
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2009
Type:
Article
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