Determination of forsythoside A in fructus forsythiae by NIR / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>Determine the content of forsythoside A in fructus forsythiae by combining near-infrared spectroscopy with the chemometrics method.</p><p><b>METHOD</b>HPLC was used as a reference method to determine the content of forsythoside A in fructus forsythiae. Multivariate calibration model based on PLS algorithm was developed to correlate the spectra and the corresponding values determined by the reference method.</p><p><b>RESULT</b>RMSECV (root-mean-square error of cross-validation) of the model for forsythoside A was 0.221 and the correlation coefficients of the calibration model was 0.969. External validation with external validation samples proved that the relative coefficient of the predicted value and the HPLC value was 0.961 and the RMSEP was 0.18.</p><p><b>CONCLUSION</b>NIRS can be used in the determination of forsythoside A, which sets up the foundation of product-line control of fructus forsythiae.</p>