Tissue culture and plant regeneration of Ardisia crenata / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>Our research studied the fast-breeding technology of Ardisia crenata sims by using tissue culture and provided the scientific foundation for industry production.</p><p><b>METHOD</b>The effects of axillary buds and plant regeneration of different basic medium, hormones and additives on induction and multiplication were studied.</p><p><b>RESULT</b>The best culture medium for the induction of axillary buds, which took the stems of A. crenate were as explants, was MS + 6-BA 0.5 mg x L(-1) + NAA 0.1 mg x L(-1), and the best medium for multiplication was MS + 6-BA 2.0 mg x L(-1) + NAA 0.1 mg x L(-1) + KT 0.5 mg x L(-1), the best medium for roots generation was 1/2MS + IBA 0.2 mg x L(-1). We also found that the roots'generation, roots rate and mean number of roots can be promoted by adding 0.2% Ac, and the most suitable ground substance was river sand-perlite-vermiculite (111) or perlite-vermiculite (11). With axillary buds and plant regeneration methode, more than 80% A. crenata sims could be regenerated integratedly.</p><p><b>CONCLUSION</b>A. crenata sims can be regenerated integratedly and breeded fast by using axillary bud proliferation technology.</p>