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Establishment of a nasopharyngeal carcinoma cell line CNE1 stably overexpressing ebv-miR-BART7 mediated by lentivirus / 南方医科大学学报
Article in Chinese | WPRIM | ID: wpr-307919
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a nasopharyngeal carcinoma (NPC) cell line CNE1-pLVTHM/BART7 with stable ebv-miR-BART7 overexpression.</p><p><b>METHODS</b>The recombinant lentivirus pLVTHM/BART7 expression plasmid was packaged into mature lentivirus by 293FT cells and used to infect CNE1 cells. Flow cytometry was employed for sorting the GFP(+) cells. The efficiency of ebv-miR-BART7 overexpression was determined using qRT-PCR.</p><p><b>RESULTS</b>The recombinant lentivirus plasmid pLVTHM/BART7 was successfully constructed and verified by PCR and sequencing. The expression of ebv-miR-BART7 in CNE1 cells infected with the lentivirus pLVTHM/BART7 was significantly increased as compared with the negative control and the blank control cells.</p><p><b>CONCLUSION</b>The recombinant lentivirus vector pLVTHM/BART7 results in high and stable expression of ebv-miR-BART7 in infected CNE1 cells, which provides a useful cell model for further studies of the role of ebv-miR-BART7 in nasopharyngeal carcinoma.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Plasmids / Humans / Carcinoma / Nasopharyngeal Neoplasms / Lentivirus / MicroRNAs / Cell Line, Tumor / Genetic Vectors / Genetics Language: Chinese Journal: Journal of Southern Medical University Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Plasmids / Humans / Carcinoma / Nasopharyngeal Neoplasms / Lentivirus / MicroRNAs / Cell Line, Tumor / Genetic Vectors / Genetics Language: Chinese Journal: Journal of Southern Medical University Year: 2011 Type: Article