Effects of Qufengtongluo Recipe on podocin mRNA expression and podocyte morphology in rats with adriamycin-induced nephropathy / 南方医科大学学报
Journal of Southern Medical University
;
(12): 244-247, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-307960
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of Qufengtongluo Recipe (QFTLR) on the expression of podocin mRNA and podocyte morphology in rats with adriamycin-induced nephropathy (AN), and explore the possible mechanism mediating the therapeutic effect of QFTLR on nephropathic proteinuria.</p><p><b>METHODS</b>SD rats were randomized into normal control group, AN model group (established by a single injection of adriamycin via the tail vein), and 3 intervention groups with QFTLR, prednisone, or benazepril treatment. After the corresponding treatments, the expression of podocin mRNA in the renal tissues was detected by RT-PCR methods, and the morphological changes of the podocytes were examined by electron microscope.</p><p><b>RESULTS</b>Compared with the normal control group, the AN model group showed significantly lowered expressions of podocin mRNA (P<0.01) with reduced podocytes and widening, fusion or even absence of the foot processes (FP). Intervention with QFTLR significantly increased the expression of podocin mRNA (P<0.01) and the number of podocytes, and obviously lessened the structural changes of the FP.</p><p><b>CONCLUSION</b>QFTLR can produce therapeutic effect against nephropathic proteinuria possibly by up-regulating the expression of podocin mRNA and improving the morphological changes of the podocytes.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Proteinuria
/
RNA, Messenger
/
Drugs, Chinese Herbal
/
Doxorubicin
/
Random Allocation
/
Rats, Sprague-Dawley
/
Intracellular Signaling Peptides and Proteins
/
Podocytes
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2011
Type:
Article
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