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Preliminary structural and functional study on a novel gene HSPCSET / 中华医学遗传学杂志
Chinese Journal of Medical Genetics ; (6): 35-39, 2009.
Article in Chinese | WPRIM | ID: wpr-307974
ABSTRACT
<p><b>OBJECTIVE</b>To characterize the structural and the functional feature of a novel gene HSPCSET isolated from human CD34+ hematopoietic stem/progenitor cells (HS/PCs).</p><p><b>METHODS</b>Bioinformatic technology was used to identify the structural features of the HSPCSET protein and perform the multiple sequence alignment. Yeast-two-hybrid system was used to identify the proteins interacting with the HSPCSET protein. After sequencing, we selected out the positive clones which had clear functions, and carried out beta-gal experiment and GST pull down assay to confirm the results. The cellular location of the HSPCSET was checked by immunofluorescence assay.</p><p><b>RESULTS</b>The HSPCSET protein belongs to a SET domain family, which is evolutionarily conserved across species. It implied that HSPCSET may have biologically important function. Using yeast-two-hybrid system, we showed that the protein sequence with SET domain might bind to 13 proteins, which involved in signaling transduction, transcriptional regulation, apoptosis, tumorigenesis, development, etc. And 4 proteins (GADD34, SIVA, DNAJ and PHF1) were confirmed by one-on-one back of the hybrid experiment, beta-gal test and GST pull down assay. When GADD34 and HSPCSET were co-transfected, they co-localized in the nucleus, suggesting a strong interaction.</p><p><b>CONCLUSION</b>The novel gene HSPCSET is likely to have biologically important function. This study provides the basis for further studies of its function in hematopoiesis and tumorigenesis.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Hematopoietic Stem Cells / Molecular Sequence Data / Proteins / Antigens, Differentiation / Chemistry / Amino Acid Sequence / Sequence Homology, Amino Acid / Protein Structure, Tertiary / Conserved Sequence / Cell Cycle Proteins Limits: Animals / Humans Language: Chinese Journal: Chinese Journal of Medical Genetics Year: 2009 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Hematopoietic Stem Cells / Molecular Sequence Data / Proteins / Antigens, Differentiation / Chemistry / Amino Acid Sequence / Sequence Homology, Amino Acid / Protein Structure, Tertiary / Conserved Sequence / Cell Cycle Proteins Limits: Animals / Humans Language: Chinese Journal: Chinese Journal of Medical Genetics Year: 2009 Type: Article