Search for Fanconi anemia/BRCA pathway defects in lymphoma cell lines / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 506-510, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-308029
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the possible relationship between defects in the FA/BRCA pathway of genomic stability and potential pathogenesis of T and B cell lymphoma.</p><p><b>METHODS</b>Nineteen cell lines derived from diverse subtypes of lymphoma for possible FA pathway defects were screened.</p><p><b>RESULTS</b>No defect in FANCD2 ubiquitination was observed. However, the FANCN protein was absent in cell lines HT and Sudhl4. This absence was correlated with enhanced MMC-induced G2 arrest, growth inhibition and high chromosomal breakage rate in both cell lines. In addition, in exon-5a of FANCN gene, a mutation of c.1769 C>T, p. A590V was found in cell line HT, but not in cell line Sudhl4.</p><p><b>CONCLUSION</b>This mutation may be the reason causing the absence of the FANCN protein expression or making the protein unstable and losing its function.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Molecular Sequence Data
/
Nuclear Proteins
/
Base Sequence
/
Signal Transduction
/
Gene Expression Regulation, Neoplastic
/
Cell Cycle
/
Chemistry
/
Mitomycin
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2008
Type:
Article
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