Identification of a novel allele human leukocyte antigen B*4609 / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 459-461, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-308039
ABSTRACT
<p><b>OBJECTIVE</b>To identify a novel human leukocyte antigen (HLA) allele.</p><p><b>METHODS</b>HLA typing was carried out with PCR-SSOP. Molecular cloning and DNA sequencing were used to identify the sequence of a potential novel allele and the difference between this new allele and other known alleles was analyzed.</p><p><b>RESULTS</b>HLA genotyping of one sample gave different results. The sequencing results showed that the HLA B alleles of the proband were B*151101 and a novel allele. The nucleotide sequence of the novel allele was different from all other known B alleles. It had one nucleotide change from the closest matching allele B*460101 at nucleotide 527 (A to T) in exon 3, resulting in an amino acid change from E (GAG) to V (GTG) at codon 176.</p><p><b>CONCLUSION</b>A novel HLA B allele was identified and officially designated as HLA B*4609 by WHO Nomenclature Committee for Factors of the HLA System in November, 2006.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Molecular Sequence Data
/
Base Sequence
/
Sequence Homology, Nucleic Acid
/
HLA-B Antigens
/
Cloning, Molecular
/
Amino Acid Substitution
/
Polymorphism, Single Nucleotide
/
Alleles
/
Allergy and Immunology
/
Genetics
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2008
Type:
Article
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