Determination of adefovir in monkey plasma by liquid chromatography-tandem mass spectrometry / 药学学报
Acta Pharmaceutica Sinica
;
(12): 120-123, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-308165
ABSTRACT
<p><b>AIM</b>To develop a sensitive and specific liquid chromatography-tandem mass spectrometry (LC/MS/MS) method for the determination of adefovir in monkey plasma.</p><p><b>METHODS</b>Adefovir and internal standard 9-(3-phosphonylmethoxypropyl) adenine were isolated from plasma by protein precipitation with methanol, then chromatographed by using a Diamonsil C18 column. The mobile phase consisted of methanol-water-formic acid (20801). Electrospray ionization (ESI) source was applied and operated in the positive ion mode. Selected reaction monitoring (SRM) mode with the transitions of m/z 274-->m/z 162 and m/z 288-->m/z 176 were used to quantify adefovir and the internal standard, respectively.</p><p><b>RESULTS</b>The linear calibration curve was obtained in the concentration range of 0.02-4.00 mg.L-1. The lower limit of quantitation was 20 micrograms.L-1. The inter- and intra-day precision (RSD) was less than 5.8%, and the accuracy (relative error) was within +/- 4.5%. The method was successfully used in a pharmacokinetic study of adefovir dipivoxil in monkeys.</p><p><b>CONCLUSION</b>The method is proved to be suitable for pre-clinical investigation of adefovir dipivoxil pharmacokinetics, which offers advantages of specificity and simple sample preparation compared with the previously reported methods.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Antiviral Agents
/
Blood
/
Pharmacokinetics
/
Biotransformation
/
Adenine
/
Chromatography, Liquid
/
Spectrometry, Mass, Electrospray Ionization
/
Organophosphonates
/
Macaca mulatta
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2003
Type:
Article
Similar
MEDLINE
...
LILACS
LIS