Effect of Saikokeishito, a Kampo medicine, on hydrogen peroxide-induced premature senescence of normal human dermal fibroblasts / 中西医结合学报
Journal of Integrative Medicine
; (12): 495-503, 2014.
Article
in En
| WPRIM
| ID: wpr-308176
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Saikokeishito (TJ-10) is a Kampo (traditional Japanese herbal) medicine, clinically used for hundreds of years in East Asia. Among its various mechanisms elucidated so far, TJ-10 inhibits the production of transforming growth factor-β1 (TGF-β1) and development of pancreatic fibrosis in vivo. Oxidative damage of normal human dermal fibroblasts (NHDFs) in the corium is a cause of human dermal senescence. Our aim was to determine whether TJ-10 protects NHDFs from premature senescence by hydrogen peroxide (H₂O₂).</p><p><b>METHODS</b>Premature senescence was induced in NHDFs by 200 μmol/L H₂O₂ for 4 h. Cell viability and the expressions of p53, AMP-activated protein kinase α1 (AMPKα1), AMPKα2, and 14-3-3 protein sigma (14-3-3 σ) were measured in NHDFs treated with TJ-10 for 48 h before exposure to H₂O₂for 4 h.</p><p><b>RESULTS</b>Cell viability after treatment with 200 μmol/L H₂O₂ for 4 h was similar (about 80%) to after pre-treatment with TJ-10. Ascorbic acid as a control did not protect NHDFs from damage by 200 μmol/L H₂O₂. Treatment with 200 μmol/L H₂O₂tended to up-regulate p53 and to down-regulate SIRT1 and AMPKα1, but had no effect on AMPKα2 and 14-3-3 σ expression. Pretreatment with TJ-10 inhibited H₂O₂-induced up-regulation of p53 and enhanced AMPKα1 expression.</p><p><b>CONCLUSION</b>It is suggested that Saikokeishito has a protective effect on oxidative stress-induced senescence of NHDFs.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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Ascorbic Acid
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Drugs, Chinese Herbal
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Down-Regulation
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Up-Regulation
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Cell Survival
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Cellular Senescence
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Cell Culture Techniques
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Dermis
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Medicine, Kampo
Limits:
Humans
Language:
En
Journal:
Journal of Integrative Medicine
Year:
2014
Type:
Article