Immunoglobulin expression in colon cancer cell line HT-29 and its biological activities / 中华肿瘤杂志

ABSTRACT

<p><b>OBJECTIVE</b>To determine the expression of immunoglobulins in HT-29 cells (an established colon cancer cell line, and explore their effect on the biological activities of the cancer cells.)</p><p><b>METHODS</b>The transcripts of variable regions of immunoglobulin heavy chains in HT-29 cells were detected by RT-PCR. Antisense CDR3 (specific to HT-29)-pIRES 1 neo vector was constructed, then transfected into HT-29 cells by electroporation. Programmed cell death and growth inhibition of HT-29 cells were detected by FCM and MTT, respectively.</p><p><b>RESULTS</b>The transcripts of Ig heavy chain (V(H) CDR3 region) were expressed in HT-29 cells. Moreover, they showed a monoclonal characteristic after being sequenced. After transfection of the antisense vector of CDR3 (specific to HT-29)-pIRES 1 neo, expression level of Ig in HT-29 cells was significantly decreased, and growth inhibition (P < 0.05) and apoptosis (P < 0.01) were induced.</p><p><b>CONCLUSION</b>These results suggest that tumor derived Ig could promote the survival and growth of tumor cells.</p>