Mechanism of augmented anti-tumor immunity in reconstituted lymphopenic mice immunized with melanoma vaccine / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 708-712, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-308458
ABSTRACT
<p><b>OBJECTIVE</b>To explore mechanisms of the augmented anti-tumor immunity observed in reconstituted lymphopenic mice (RLM) receiving melanoma vaccination.</p><p><b>METHODS</b>The study is to investigate the anti-tumor immunity of tumor vaccination during early immune reconstitution period following irradiation and cyclophosphamide (CY)-induced lymphopenia. Lymphopenic mice were subsequently reconstituted with naive splenocytes from syngeneic mice and immunized with irradiated melanoma cells F10 (irradiation experiment) and GM-CSF-modified D5 melanoma cells (D5-G6) (CY experiment). Controls included normal C57BL/6 mice receiving the corresponding vaccination, un-immunized naive mice and RLM. 8 - 10 days after vaccination, tumor vaccine draining lymph nodes (TVDLN) were harvested and phenotyped by FACS analysis. T cells purified from TVDLN were stimulated with anti-CD3 and anti-TCRbeta and proliferation was assessed by [(3)H]-TdR incorporation and FACS assay was performed for CD69 expression.</p><p><b>RESULTS</b>The augmented anti-tumor immunity correlated with a significant increase in the percentage of T cells with activation/memory phenotype in the TVDLN of vaccinated RLM, compared to that of the controls. There was also a significant increase in the density of DCs in TVDLNs. The activation threshold of T cells generated from vaccinated RLM was significantly decreased, resulting in markedly enhanced proliferating capability upon anti-CD3 stimulation.</p><p><b>CONCLUSION</b>This study suggests that the augmented anti-tumor immunity observed in vaccinated RLM is due to down regulated activation threshold of T cells during lymphopenia-driven T cell proliferation, which may in turn facilitate the breaking down of immune tolerance to weak tumor antigens upon vaccination with tumor cell vaccines.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Therapeutics
/
Melanoma, Experimental
/
T-Lymphocytes
/
Whole-Body Irradiation
/
Cancer Vaccines
/
Cyclophosphamide
/
Allergy and Immunology
/
Lymph Nodes
/
Lymphopenia
/
Mice, Inbred C57BL
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2005
Type:
Article
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