Activating effect of citrus flavonoids on neuromedin U2 receptor and analysis on siRNA interference / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3462-3466, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-308634
ABSTRACT
<p><b>OBJECTIVE</b>To screen out active substances on Neuromedin U2 receptor (NMU2R) by using stable NMU2R cell lines and negative cell lines and analyzing siRNA interference.</p><p><b>METHOD</b>NMU2R cells were used to observe the activating effect of nine nine citrus flavonoids on NMU2R cell. Afterwards, false-positive interference of citrus flavonoids that showed higher activating effect was eliminated by using negative cells and analyzing the efficiency of siRNA interference.</p><p><b>RESULT</b>Hesperidin and nobiletin contained in citrus flavonoids were found to effectively activate NMU2R. The efficacy, EC50 and potency values of hesperidin were 4.688, 318.970 micromol x L(-1) and 200.933 micromol x L(-1), while the efficacy, EC50 and potency values of nobiletin were 4.758, 5.832 micromol x L(-1) and 3.124 micromol x L(-).</p><p><b>CONCLUSION</b>Hesperidin and nobiletin contained in citrus flavonoids can activate NMU2R. Nobiletin shows such a low EC50 that it has medicinal value.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Flavonoids
/
Plant Extracts
/
Gene Expression
/
Cell Line
/
Chemistry
/
Citrus
/
Receptors, Neurotransmitter
/
RNA, Small Interfering
/
RNA Interference
Limits:
Humans
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2012
Type:
Article
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