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Genetic diversity of Atractylodes macrocephala by ISSR / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 3381-3385, 2012.
Article in Chinese | WPRIM | ID: wpr-308650
ABSTRACT
<p><b>OBJECTIVE</b>To assess the genetic diversity of Atractylodes macrocephala in 12 cultivated populations and 3 wild populations.</p><p><b>METHOD</b>The molecular marker technique ISSR was used to investigate the genetic diversity of 365 samples from 15 populations in Zhejiang, Anhui and Hebei Provinces. Data was analyzed by POPGEN 32, and a cluster diagram was presented by UPGMA.</p><p><b>RESULT</b>One hundred and two amplified fragments were obtained using 13 ISSR primers. 94 polymorphic loci were identified as 92.16%. Nei's genetic diversity index (He) was 0.406 5, Shannon diversity index (I) was 0.590 3, and the coefficient of gene differentiation (Gst) was 0.202 5. The genetic similarity coefficient among the populations ranged from 0.690 7 to 0.960 5 in an average of 0. 825 6. No significant difference was found among the populations based on the clustering analysis.</p><p><b>CONCLUSION</b>There is a low level of genetic differentiation in higher genetic diversity of the A. macrocephala populations. No obvious regional or species diversities were identified. This is consistent with current status of cultivation and gerplasm circulation in A. macrocephala.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Genetic Variation / China / Classification / Microsatellite Repeats / Atractylodes / Amplified Fragment Length Polymorphism Analysis / Genetics Country/Region as subject: Asia Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2012 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Genetic Variation / China / Classification / Microsatellite Repeats / Atractylodes / Amplified Fragment Length Polymorphism Analysis / Genetics Country/Region as subject: Asia Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2012 Type: Article