Study on norcantharidin-induced apoptosis in SMMC-7721 cells through mitochondrial pathways / 中国结合医学杂志
Chinese journal of integrative medicine
;
(12): 448-452, 2010.
Article
in English
| WPRIM
| ID: wpr-308738
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the mechanism of norcantharidin (NCTD)-induced SMMC-7721 hepatoma cell apoptosis.</p><p><b>METHODS</b>SMMC-7721 cell growth inhibition was measured by the MTT method. Apoptosis was detected by Annexin V/propidium iodide staining. The mitochondrial membrane potential was measured by flow cytometry. Western blot analysis was used to evaluate the level of cytochrome c, caspase-3, AIF, Bcl-2 and Bax expression.</p><p><b>RESULTS</b>NCTD inhibited SMMC-7721 cell growth in a time- and dose-dependent manner. The cells treated with NCTD showed the loss of mitochondrial membrane potential. The activities of caspase-3, cytochrome c, AIF, and Bax were up-regulated after NCTD treatment at different doses. The expression of Bcl-2 was decreased after treatment with NCTD.</p><p><b>CONCLUSIONS</b>NCTD could induce SMMC-7721 cell apoptosis. The activation of the mitochondrial pathway was involved in the process of NCTD-induced SMMC-7721 cell apoptosis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Blotting, Western
/
Apoptosis
/
Bridged Bicyclo Compounds, Heterocyclic
/
Cell Line, Tumor
/
Cytochromes c
/
Apoptosis Inducing Factor
/
Bcl-2-Associated X Protein
/
Caspase 3
Limits:
Humans
Language:
English
Journal:
Chinese journal of integrative medicine
Year:
2010
Type:
Article
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