Protective effects of lidocaine on hyperoxia-exposed type Ⅱalveolar epithelial cells from premature rats / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 313-316, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-308803
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of lidocaine on apoptosis and proliferation of hyperoxia-exposed type Ⅱalveolar epithelial cells (AECⅡ) from premature rats.</p><p><b>METHODS</b>Primary cultured AEC Ⅱ isolated from premature rats were randomly divided into four groups air, air+ lidocaine (20 μg/mL), 95%O2/5%CO2, and 95%O2/5%CO2+ lidocaine. The cells in each group were collected 24 hrs after culture in ordinary incubators (37℃,5%CO2). The proliferation and apoptosis of AEC Ⅱ were detected by flow cytometry. Protein levels of proliferating cell nuclear antigen (PCNA) were measured by Western blot.</p><p><b>RESULTS</b>The apoptosis rate of AECⅡincreased, the percentages of G2/M and S phase cells decreased and the protein levels of PCNA decreased significantly in the group exposed to 95%O2/5%CO2 compared with the group exposed to air (P<0.01). Lidocaine treatment decreased apoptosis rate of AECⅡ, increased percentage of G2/M and S phase cells, and increased protein levels of PCNA.</p><p><b>CONCLUSIONS</b>Hyperoxia can increase apoptosis and inhibit proliferation of AECⅡ in premature rats. Lidocaine may have protective effects against the AECⅡ injuries.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Pulmonary Alveoli
/
Cell Cycle
/
Rats, Sprague-Dawley
/
Apoptosis
/
Proliferating Cell Nuclear Antigen
/
Hyperoxia
/
Cytoprotection
/
Epithelial Cells
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2011
Type:
Article
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