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Efficacy of HSV-tk/GCV system on human laryngeal squamous cell cancer in vitro / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery ; (12): 374-376, 2006.
Article in Chinese | WPRIM | ID: wpr-308894
ABSTRACT
<p><b>OBJECTIVE</b>Efficacy of HSV-tk/GCV system antitumor effects was assessed on human laryngeal cancer cell line Hep-2 in vitro. To assess the HSV-tk/CGV system whether has an antitumour effect on human laryngeal squamous cell cancer Hep-2 in vitro. The mechanisms of cytotoxity were also assessed.</p><p><b>METHODS</b>Hep-2 cells were transfected with HSV-tk gene by lipofection. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the HSV-tk gene expression. MTT was utilized to test for the cytotoxicity of this system. The cell-circle arrest and apoptosis were analyzed by flowcytometry assay.</p><p><b>RESULTS</b>HSV-tk gene transfected cells demonstrated obvious cytoreductivity followed by ganciclovir (GCV) administration and this cytoreductivity showed partial GCV dose-independent. HSV-tk gene transfected cells demonstrated obvious s-phase arrest, no apoptosis and necrosis occurred.</p><p><b>CONCLUSIONS</b>The HSV-tk/GCV system can inhabit the growth of Hep-2 cells effectively. S-phase arrest perhaps is the main reason that leads to the cell inhibition in our study. HSV-tk/GCV system has potential antitumor effects for the future clinical practice.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Therapeutics / Thymidine Kinase / Carcinoma, Squamous Cell / Transfection / Genetic Therapy / Ganciclovir / Laryngeal Neoplasms / Simplexvirus / Cell Line, Tumor / Genes, Transgenic, Suicide Limits: Humans Language: Chinese Journal: Chinese Journal of Otorhinolaryngology Head and Neck Surgery Year: 2006 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Therapeutics / Thymidine Kinase / Carcinoma, Squamous Cell / Transfection / Genetic Therapy / Ganciclovir / Laryngeal Neoplasms / Simplexvirus / Cell Line, Tumor / Genes, Transgenic, Suicide Limits: Humans Language: Chinese Journal: Chinese Journal of Otorhinolaryngology Head and Neck Surgery Year: 2006 Type: Article