Affection of Wnt/beta-catenin in Titanium Particles Challenged Osteoblasts / 대한정형외과연구학회지
Journal of Korean Orthopaedic Research Society
;
: 35-42, 2010.
Article
in Korean
| WPRIM
| ID: wpr-30900
ABSTRACT
PURPOSE:
The intracellular mechanisms that lead to periprosthetic osteolysis including impaired bone forming activity of osteoblast remain incompletely characterized. To determine the possibility that Ti-particles play a role to regulate Wnt/beta-catenin signaling pathway in impaired osteogenesis, we analyzed the stability of beta-catenin and the transcriptional changes of regulators for Wnt/beta-catenin signaling pathway in MC3T3-E1 osteoblast cells. MATERIALS ANDMETHODS:
Ti-particles were prepared by sterilizing and counted on the microscopy. Transcriptional changes of OPG, RANKL, LRP5, LRP6, DKK1 and sFRP2 were determined by real-time RTPCR. Protein level of beta-catenin and GSK3beta was detected using Western blotting and immunofluorescence staining.RESULTS:
After 4 hours of treatment of Ti-particles, OPG/RANKL mRNA ratio was significantly decreased. And also, decreased protein levels of beta-catenin and phospho-GSK3beta were detected. Using immunofluorescence stain, it was confirmed that Ti-particles suppressed nucleus staining of beta-catenin induced by Wnt3a conditioned medium. The results of real-time RT-PCR showed reduced level of LRP5 and LRP6 transcripts, and induced level of DKK1 and sFRP2 transcripts by challenging of Ti-particlesCONCLUSION:
Our report suggests that Ti-particles may play a crucial role in the regulation of Wnt/beta-catenin signaling pathway in osteoblast through the transcriptional changes of membrane receptors and extracellular inhibitors for Wnt.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Osteogenesis
/
Osteolysis
/
Titanium
/
RNA, Messenger
/
Blotting, Western
/
Fluorescent Antibody Technique
/
Culture Media, Conditioned
/
Glycogen Synthase Kinase 3
/
Beta Catenin
Language:
Korean
Journal:
Journal of Korean Orthopaedic Research Society
Year:
2010
Type:
Article
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