Preliminary research on the expression of sclerostin mediated by bone morphogenetic protein 2 in cementoblast / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 244-247, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-309144
ABSTRACT
<p><b>OBJECTIVE</b>This research explores the regulatory role of bone morphogenetic protein 2 (BMP2) in the expression of sclerostin in OCCM-30 cementoblast.</p><p><b>METHODS</b>OCCM-30 cementoblasts were treated with 50 and 100 ng · mL⁻¹ BMP2 for 3, 5, and 7 days. SOST mRNA was detected by real-time quantitative polymerase chain reaction (RT-PCR). Western blot analysis was employed to detect the sclerostin levels in the nucleus. Five groups were prepared for the experiments control, BMP2, BMP2+dorsomorphin, BMP2+SB202190, and BMP2+PD98059. OCCM-30 was pretreated with BMP2 for 3 and 5 days, and then the sclerostin and SOST mRNA levels were measured.</p><p><b>RESULTS</b>RT-PCR and Western blot analyses showed that BMP2 upregulated the expression of SOST in a concentration-dependent manner. SOST expression increased with time (P < 0.05). Moreover, sclerostin levels of BMP2+dorsomorphin, BMP2+SB202190, and BMP2+PD98059 groups were lower than that of the BMP2 group, and the sclerostin level in BMP2+dorsomorphin group was lowest (P < 0.05).</p><p><b>CONCLUSION</b>The upregulation of SOST by BMP2 in OCCM-30 is mainly mediated by the BMP2/Smad signal pathway.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Signal Transduction
/
Genetic Markers
/
Up-Regulation
/
Blotting, Western
/
Bone Morphogenetic Proteins
/
Dental Cementum
/
Bone Morphogenetic Protein 2
/
Metabolism
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2016
Type:
Article
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