Protective effect of maternal immunoglobulin G against NMDA-induced neurotoxicity on hippocampus neurons / 中华儿科杂志
Chinese Journal of Pediatrics
;
(12): 374-376, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-309197
ABSTRACT
<p><b>OBJECTIVE</b>To investigate possible protective effect of maternal immunoglobulin G (IgG) against N-methyl-D-aspartate-mediated neurotoxicity on primary-cultured rat hippocampal neurons and the mechanism of the effect.</p><p><b>METHODS</b>An in vitro system had been developed for the study of hippocampal neurons. Intracellular lactic dehydrogenase (LDH) release was used as a marker to measure the rates of neuronal damage. The cells were stained with Trypan blue to measure the rate of neuronal death.</p><p><b>RESULTS</b>N-methyl-D-aspartate (NMDA) at a concentration of 50 micromol/L resulted in increased release of LDH and the cell mortality (P < 0.01, respectively). Maternal IgG of different concentration (10 mg/L, 100 mg/L) inhibited NMDA-induced intracellular LDH release (P < 0.01, respectively) and cell mortality (P < 0.05, 0.01, respectively), and larger dose had stronger effect (P < 0.05).</p><p><b>CONCLUSIONS</b>Maternal IgG had protective effect on primary-cultured rat hippocampal neurons injured by NMDA and the effect was dose-dependent.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Organ Culture Techniques
/
Pathology
/
Pharmacology
/
Immunoglobulin G
/
Cell Survival
/
Cells, Cultured
/
N-Methylaspartate
/
Cell Death
/
Rats, Wistar
/
Excitatory Amino Acid Agonists
Limits:
Animals
/
Pregnancy
Language:
Chinese
Journal:
Chinese Journal of Pediatrics
Year:
2006
Type:
Article
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