The molecular mechanisms of curcuma wenyujin extract-mediated inhibitory effects on human esophageal carcinoma cells in vitro / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 1219-1222, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-309291
ABSTRACT
<p><b>OBJECTIVE</b>To study the molecular mechanisms of Curcuma Wenyujin extract-mediated inhibitory effects on human esophageal carcinoma cells.</p><p><b>METHODS</b>The Curcuma Wenyujin extract was obtained by supercritical carbon dioxide extraction. TE-1 cells were divided into 4 groups after adherence. 100 microL RMPI-1640 culture medium containing 0.1% DMSO was added in Group 1 as the control group. 100 microL 25, 50, and 100 mg/L Curcuma Wenyujin extract complete culture medium was respectively added in the rest 3 groups as the low, middle, and high dose Curcuma Wenyujin extract groups. The effects of different doses of Curcuma Wenyujin extract (25, 50, and 100 mg/L) on the proliferation of human esophageal carcinoma cell line TE-1 in vitro were analyzed by MTT assay. The gene expression profile was identified by cDNA microarrays in esophageal carcinoma TE-1 cells exposed to Curcuma Wenyujin extract for 48 h. The differential expression genes were further analyzed by Gene Ontology function analysis.</p><p><b>RESULTS</b>Compared with the control group, MTT results showed that Curcuma Wenyujin extract significantly inhibited the proliferation of TE-1 cells in a dose-dependent manner (P<0.05). The expression level of 88 genes changed with significance, including 66 up-regulation genes and 22 down-regulation genes. Gene Ontology analysis indicated the genes coding for proteins was involved in signal transduction (6), cell cycle (8), apoptosis (14), and cell differentiation (10).</p><p><b>CONCLUSIONS</b>The Curcuma Wenyujin extract could inhibit the growth of human esophageal carcinoma cell line TE-1 in vitro. The molecular mechanisms might be associated with regulating genes expressions at multi-levels.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Esophageal Neoplasms
/
Plant Extracts
/
Carcinoma
/
Gene Expression Regulation, Neoplastic
/
Apoptosis
/
Curcuma
/
Cell Line, Tumor
/
Cell Proliferation
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2012
Type:
Article
Similar
MEDLINE
...
LILACS
LIS