Concurrent Assay for Four Bacterial Species Including Alloiococcus Otitidis in Middle Ear, Nasopharynx and Tonsils of Children with Otitis Media with Effusion: A Preliminary Report
Clinical and Experimental Otorhinolaryngology
;
: 81-85, 2012.
Article
in English
| WPRIM
| ID: wpr-30934
ABSTRACT
OBJECTIVES:
To detect the prevalences of Alloiococcus otitidis, as well as Haemophilus influenzae, Streptococcus pneumoniae, and Moraxella catarrhalis in children with chronic otitis media with effusion (OME) and to simultaneously investigate the colonization of these bacteria in the nasopharynx and palatine tonsils of these patients.METHODS:
The study included 34 pediatric patients with OME, and 15 controls without OME. In the study group, A. otitidis, H. influenzae, S. pneumoniae, and M. catarrhalis were investigated in the samples obtained from middle ear effusions (MEE), nasopharyngeal swabs (NPS) and tonsillar swabs (TS), using multiplex polymerase chain reaction (PCR) and conventional culture methods. Only the samples obtained from NPS and TS were studied with the same techniques in the control group.RESULTS:
A. otitidis was isolated only in MEE and only with multiplex PCR method. A. otitidis, S. pneumoniae, M. catarrhalis, H. influenzae were identified in 35%, 8.8%, 8.8%, and 2.9%, respectively, in 34 MEE. A. otitidis was not isolated in NPS or TS of the study and the control groups.CONCLUSION:
The prevalence of A.otitidis is high in children with OME and A.otitidis doesn't colonize in the nasopharynx or tonsil.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Otitis
/
Otitis Media
/
Pneumonia
/
Streptococcus pneumoniae
/
Bacteria
/
Palatine Tonsil
/
Otitis Media with Effusion
/
Nasopharynx
/
Haemophilus influenzae
/
Polymerase Chain Reaction
Type of study:
Prevalence study
Limits:
Child
/
Humans
Language:
English
Journal:
Clinical and Experimental Otorhinolaryngology
Year:
2012
Type:
Article
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