Loss of heterozygosity on chromosome 10 is associated with bone metastasis of human prostate cancer / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the cytogenetic mechanism of bone metastasis of human prostate cancer (PCa).</p><p><b>METHODS</b>We analyzed chromosome variation by comparative genomic hybridization in 18 patients with prostate cancer to determine the chromosome variants associated with bone metastasis, and focused on 7 microsatellite sites on chromosome 10 for the detection of the loss of heterozygosity (LOH) by PCR-based microsatellite polymorphism analysis.</p><p><b>RESULTS</b>In the 11 samples with bone metastasis, the variation rate of chromosome 10 was 90.9% (10/11), significantly higher than that of the others (P < 0.01). A much higher LOH frequency was observed at the 7 microsatellite loci on chromosome 10 and the highest located in 10q24. 2-q25.3 (D10S1693-D10S587) in the PCa patients with bone metastasis.</p><p><b>CONCLUSION</b>There is a high-frequency LOH region in 10q24. 2-q25.3 (D10S1693-D10S587) on chromosome 10 in PCa patients with bone metastasis, which may be potentially involved in PCa progression and specific bone metastasis.</p>