Comparison between Real-Time PCR and Agarose Gel Electrophoresis for DNA Quantification / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 217-222, 2006.
Article
in Korean
| WPRIM
| ID: wpr-30977
ABSTRACT
BACKGROUND:
Real-time polymerase chain reaction (PCR) is generally regarded as a very accurate and time-saving method, but it is expensive to run. We evaluated the reliability of an inexpensive and a researcher-friendly gel electrophoresis-based PCR method for the quantification of mRNA, and the results were compared with those obtained by real-time PCR.METHODS:
We compared the results of relative quantification for MMP-1 measured by real-time PCR and by ethidium bromide stained-agarose gel electrophoresis after end-point PCR.RESULTS:
There was significant but very weak correlation between real-time PCR and end-point PCR for relative quantification of MMP-1 (r=0.16, P<0.01).CONCLUSIONS:
Our results suggest that the use of the gel electrophoresis-based end-point PCR is inappropriate for quantifying mRNA. Therefore, in order to confirm the result of relative quantification by end-point PCR, the newly established real-time PCR method or northern hybridization should be applied.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sepharose
/
DNA
/
RNA, Messenger
/
Polymerase Chain Reaction
/
Electrophoresis
/
Electrophoresis, Agar Gel
/
Ethidium
/
Real-Time Polymerase Chain Reaction
Language:
Korean
Journal:
The Korean Journal of Laboratory Medicine
Year:
2006
Type:
Article
Similar
MEDLINE
...
LILACS
LIS