Aberrant expression of sperm protein 17 enhances migration of ovarian cancer cell line HO-8910 / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore the effects of aberrant expression of sperm protein 17 (Sp17) on the migration of the ovarian cancer cell line HO-8910.</p><p><b>METHODS</b>The recombinant plasmid pEGFP-Sp17 containing Sp17 and enhanced green fluorescent protein gene was transfected into the human ovarian cancer cell line HO-8910 with Lipofectamine 2000. The expression of Sp17 was examined by RT-PCR and Western blot, and the cell migratory capability detected by Transwell chamber assays.</p><p><b>RESULTS</b>Sp17 was expressed as a fusion protein with EGFP after transfected. There was a significant difference in the migratory cell number of the transfected and the control cells (156.6 +/- 14.9/HP vs 39.3 +/- 8.53/HP, P < 0.05).</p><p><b>CONCLUSION</b>The aberrant expression of Sp17 greatly enhances the migration of ovarian cancer cells.</p>