Research and development of a piezoelectric immunosensor for detecting Treponema pallidum / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 1215-1218, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-309918
ABSTRACT
We utilized polyethylenimine (PEI) and Glu solution to immobilize the antibody of Treponema Pallidum on the silver electrode of piezoelectric quartz crystal and detect the standard antigen solution at different concentration. The antibody keeps the intrinsic Y type molecular structure revealed by AFM. The resonant range of the sensor is between 5 x 10(-5) - 1.25 x 10(-4) g/mL and the correlation coefficient is 0.9976, the optimal resonance pH is 7.5. We found the sensor having good selectivity in comparison with the method using BSA. At last, a discussion on the reproducibility of the sensor is presented.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polyethyleneimine
/
Quartz
/
Treponema pallidum
/
Immunoassay
/
Biosensing Techniques
/
Allergy and Immunology
/
Equipment Design
/
Methods
/
Microelectrodes
/
Antibodies, Bacterial
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2005
Type:
Article
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