Study on the conditions of high density fermentation for the engineering bacterial strain with RGD spider silk protein gene / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 1206-1209, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-309920
ABSTRACT
Spider silk becomes the protein fibroin with excellent character in the natural world because of its unique mechanical properties. The genetic engineering technique is an effective method to procure the spider silk protein. In order to obtain RGD-spider silk protein gene recombinant engineering strain pNSR-16 on a regular scale, we determine the optimal conditions of the growth and expression of pNSR-16 through culture in laboratory, and based on these, study the fed-batch high density culture. We control the flow of carbon-nitrogen, the dissolved oxygen concentration and the cell specific growth rate, which make the final cell density and concentration of recombinant protein of total protein arrive at OD600 57.15 and 20.8%, respectively.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oligopeptides
/
Recombination, Genetic
/
Bacteria
/
Recombinant Proteins
/
Protein Engineering
/
Genetic Engineering
/
Cloning, Molecular
/
Fermentation
/
Fibroins
/
Genetics
Limits:
Animals
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2005
Type:
Article
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