The role and mechanisms of cyclooxygenase-2 inhibitors on the proliferation of hepatic stellate cell / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 346-349, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-310089
ABSTRACT
<p><b>OBJECTIVES</b>To observe the effects of NS-398 on proliferation of hepatic stellate cells (HSCs) in vitro, and to investigate the possible molecule mechanism.</p><p><b>METHODS</b>HSCs were incubated with different concentrations of NS-398. The effects of NS-398 on cell proliferation was detected by MTT colormetric assay. The cell cycle of HSCs was analyzed by Flow Cytometry (FCM), cyclooxygenase-2 (COX-2) and proliferating cell nuclear antigen (PCNA) proteins in HSCs were detected by immunocytochemistry.</p><p><b>RESULTS</b>Administration of 20-160 micromol/L NS-398 significantly inhibited HSCs proliferation in dose-dependent manner compared with the control group (P less than 0.01). After treated with NS-398 at concentrations of 90, 120, and 150 micromol/L for 48 h, the number of HSCs in G(2)/M phase increased and the number of HSCs in G(0)/G(1) phase decreased (P less than 0.05); Incubated with 120 micromol/L NS-398 for 48 h, percentage of masculine cell of PCNA was 28.91%+/-0.11%, which was significantly lower than that of the control group (85.99%+/-0.13%) (P less than 0.01). Percentage of masculine cell of COX-2 was 13.80%+/-0.43%, which was not significantly different from that of the control group (14.07%+/-0.59%) (P more than 0.05).</p><p><b>CONCLUSIONS</b>NS-398 could inhibit the proliferation of HSC-T6 and arrest HSCs in G2/M phase. Down-regulation of PCNA protein may partially accounted for the proliferation inhibition effect on HSCs induced by NS-398.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Sulfonamides
/
Cell Cycle
/
Cell Line
/
Cyclooxygenase Inhibitors
/
Apoptosis
/
Proliferating Cell Nuclear Antigen
/
Cell Proliferation
/
Dose-Response Relationship, Drug
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2009
Type:
Article
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