Role of focal adhesion kinase in the hypoxia-induced invasion of SMMC-7721 cells / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 284-287, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-310109
ABSTRACT
<p><b>OBJECTIVE</b>To study focal adhesion kinase (FAK) expression in hypoxia-stressed SMMC-7721 cells and the role of FAK expression in the hypoxia-induced invasion of SMMC-7721 cells.</p><p><b>METHODS</b>SMMC-7721 cells were cultured in 21% O2 or 1% O2. FAK expression was determined by Western blot. The siRNA expression vector pshRNA-FAK targeting to FAK and the control vector pGensil-2 were transfected into SMMC-7721 cells. The hypoxia-induced migration and invasion ability of SMMC-7721 cells transfected with pshRNA-FAK were analyzed. In normoxia, invasion of SMMC-7721 cells transfected with pcDNA3-FAK was analyzed.</p><p><b>RESULTS</b>The expression of FAK was increased significantly in SMMC-7721 cells 24 h after hypoxia stress (P<0.01). The level of FAK protein was decreased by 74.6%+/-5.1% after the pshRNA-FAK transfection in normoxia and hypoxia. The migration and invasion of SMMC-7721 cells was increased in 1% O2 (P<0.01). However, the migration and invasion of SMMC-7721 cells transfected with pshRNA-FAK was decreased in 1% O2 (P<0.05). Overexpression of FAK significantly stimulated the invasion of SMMC-7721 cells.</p><p><b>CONCLUSION</b>Up-regulation of FAK may play an important role in the invasion of SMMC-7721 cells induced by hypoxia.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Plasmids
/
Transfection
/
Gene Expression Regulation, Enzymologic
/
Cell Movement
/
Blotting, Western
/
Carcinoma, Hepatocellular
/
RNA, Small Interfering
/
Cell Line, Tumor
/
Focal Adhesion Protein-Tyrosine Kinases
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2009
Type:
Article
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