Cordyceps sinensis polysaccharide enhances apoptosis of HL-60 cells induced by triptolide / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 158-162, 2009.
Article
in Zh
| WPRIM
| ID: wpr-310374
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of polysaccharide fraction of Cordyceps sinensis (PSCS) on triptolide (TPL)-induced apoptosis in the HL-60 cells and the involved molecular mechanism.</p><p><b>METHODS</b>The cultured leukemia HL-60 cells were divided into three groups: control group, TPL group (cells were treated with 5 ng/ml TPL only), and PSCS+TPL cells group (cells treated with 5 ng/ml TPL and 100 microg/ml or 200 microg/ml PSCS for 18 h). Cell viability was tested by MTT assay and apoptotic cells were quantitatively measured by flow cytometry with Annexin V/PI double stain.The expressions of Caspase-3, 6, 7, 9 and NF-kappa B proteins were tested by Western blot.</p><p><b>RESULT</b>MTT assay showed that different concentrations of PSCS inhibited the cell viability. Flow cytometry indicated that TPL markedly increased the apoptosis rate of the HL-60 cells, and PSCS enhanced the apoptosis in a dose-dependent manner. Western blot showed that TPL did not inhibit the expression of the Caspase-3, 6, 7, 9 and NF-kappa B proteins, and when cells were treated with PSCS, the expression of proteins decreased with the PSCS concentration rising.</p><p><b>CONCLUSION</b>PSCS can enhance TPL-induced apoptosis in HL-60 cells and inhibit the expression of NF-kappa B and Caspase 3,6,7,9,which might be the possible signaling pathway of inducing apoptosis.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Phenanthrenes
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Polysaccharides
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Chemistry
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NF-kappa B
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Apoptosis
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HL-60 Cells
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Caspases
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Cordyceps
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Diterpenes
Limits:
Humans
Language:
Zh
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2009
Type:
Article