Aspirin-PEI-beta-CyD as a novel non-viral vector for gene transfer / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 46-52, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-310392
ABSTRACT
<p><b>OBJECTIVE</b>To develop a novel non-viral gene delivery vector based on PEI-beta-CyD as backbone modified with aspirin, and to identify its physicochemical characters.</p><p><b>METHODS</b>1, 1-carbonyldiimidazole (CDI) was used to bind aspirin onto PEI-beta-CyD to form PEI-beta-CyD-ASP. (1)H-NMR, FT-IR, UV and XRD were used to confirm the polymer structure. The ability of condensation was demonstrated by gel retardation assay. MTT assay was used to test the cell viability in B16, Hela and A293 cell lines. Transfection efficiency of the polymer was tested in B16 cells.</p><p><b>RESULT</b>The structure of PEI-beta-CyD-ASP was confirmed by (1)H-NMR, FT-IR, UV and XRD, which efficiently condensed plasmid DNA at the N/P ratio of 4. The copolymer showed low cytotoxicity and high transfection efficiency in B16 cells.</p><p><b>CONCLUSION</b>The synthesized aspirin-PEI-beta-CyD might be a potential gene delivery vector.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polyethyleneimine
/
Genetic Therapy
/
Cell Line
/
Chemistry
/
Aspirin
/
Gene Transfer Techniques
/
Beta-Cyclodextrins
/
Methods
Limits:
Humans
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2009
Type:
Article
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