Effect of Porphyromonas gingivalis lipopolysaccharide on induced secretion of inflammatory cytokines by different cell lines / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 622-628, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-310404
ABSTRACT
<p><b>OBJECTIVE</b>To determine the effect of Porphyromonas gingivalis lipopolysaccharide (LPS) on induced secretion of inflammatory cytokines by different cell lines.</p><p><b>METHODS</b>LPS of P. gingivalis strain ATCC33277 (Pg-LPS) was extracted with phenol-water method, and identified by Limulus test and infrared spectrum analysis. KB, HGF-1 and THP-1 cells were treated with Pg-LPS of different concentrations and time duration, a commercial LPS of E.coli strain O111B4 (E-LPS) was used as the control. TNF-alpha, IL-1beta, IL-6 and IL-8 levels in culture supernatants were measured by quantitative ELISA.</p><p><b>RESULT</b>The minimal dosages of both Pg-LPS and E-LPS to solidify Limulus agents were 15 ng/ml and their infrared spectrums were similar. With the treatment of Pg-LPS or E-LPS, the TNF-alpha and IL-1beta levels secreted by HGF-1 cells were remarkably increased with a single perk (P<0.01) while a continuous enhancement of secretion by THP-1 cells was observed (P<0.01). Either Pg-LPS or E-LPS stimulated HGF-1 or THP-1 cells to continuously increase the secretion of IL-6 (P<0.01). Both Pg-LPS and E-LPS induced IL-8 secretion by THP-1 cells (P<0.01), but only Pg-LPS showed the similar effect on HGF-1 cells (P<0.01). Neither Pg-LPS nor E-LPS induced KB cells to secrete inflammatory cytokines.</p><p><b>CONCLUSION</b>Pg-LPS can promote target cells to increase their secretion of inflammatory cytokines. KB cells can not be used as the target cell to determine inflammation-causing effect of LPS.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Cell Line
/
Chemistry
/
Lipopolysaccharides
/
Interleukin-8
/
Interleukin-6
/
Tumor Necrosis Factor-alpha
/
Porphyromonas gingivalis
/
Bodily Secretions
/
Cell Biology
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2008
Type:
Article
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