5-Lipoxygenase/cysteinyl leukotriene pathway is not involved in injury of rat C6 glioma cells induced by oxygen-glucose deprivation / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 456-462, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-310431
ABSTRACT
<p><b>OBJECTIVE</b>To determine whether oxygen-glucose deprivation (OGD) induces C6 cell injury, and whether 5-lipoxygenase (5-LOX)/cysteinyl leukotriene (CysLT) pathway is involved in OGD-induced injury.</p><p><b>METHODS</b>After OGD treatment and recovery for various durations, the viability of C6 cells was determined, and the effects of 5-LOX inhibitors and CysLT receptor antagonists were investigated. Intracellular distribution of 5-LOX protein was detected by immunocytochemistry, and the mRNA expressions of CysLT1 and CysLT2 receptors were detected by RT-PCR. The effect of leukotriene D(4) (LTD(4)) on C6 cells was also investigated.</p><p><b>RESULT</b>OGD for 4-8 h followed by recovery for 24-72 h significantly induced C6 cell injury. Neither 5-LOX inhibitors nor CysLT receptor antagonists inhibited OGD-induced injury. OGD did not induce 5-LOX translocation into the nuclear membrane. C6 cells highly expressed CysLT(2) receptor, but the expression of CysLT1receptor was much weaker; the expression was not affected by OGD. In addition, LTD(4) did not affect C6 cells significantly.</p><p><b>CONCLUSION</b>OGD can induce C6 cell injury, but 5-LOX/CysLT pathway might be not involved in OGD-induced injury.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Arachidonate 5-Lipoxygenase
/
Tumor Cells, Cultured
/
Signal Transduction
/
Cell Hypoxia
/
Receptors, Leukotriene
/
Glioma
/
Glucose
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2008
Type:
Article
Similar
MEDLINE
...
LILACS
LIS