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LRIG3 gene regulates biological activity of GL15 cell line / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences ; (6): 444-450, 2008.
Article in Chinese | WPRIM | ID: wpr-310433
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of leucine-rich repeats and immunoglobulin-like domains 3 (LRIG3) on the biological activity of glioblastoma cell line GL15.</p><p><b>METHODS</b>Glioblastoma GL15 cells were cultured and transfected with LRIG3-EGFP plasmid. The location of LRIG3 in GL15 cells was observed with confocal microscopy. The proliferation and invasiveness of GL15 cells were detected with methyl thiazolyl tetrazolium (MTT) and Transwell methods respectively; the expression of epidermal growth factor receptor (EGFR) and LRIG3 mRNA and protein were detected with reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot respectively.</p><p><b>RESULT</b>After transfection with the plasmid LRIG-EGFP, LRIG3 fusion protein was found in cytoplasm of GL15 cells and cell proliferative and invasiveness were reduced. The expression of EGFR and LRIG3 varied with the duration of EGF treatment (100 ng/ml) the expression of EGFR decreased while the expression of LRIG3 increased as time prolonged.</p><p><b>CONCLUSION</b>LRIG3 can inhibit the proliferation and invasiveness of glioblastoma cells and may be used as a target gene in gene therapy of glioblastoma.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Plasmids / Recombinant Fusion Proteins / Brain Neoplasms / RNA, Messenger / Tumor Cells, Cultured / Transfection / Glioblastoma / Cell Proliferation / Epidermal Growth Factor Limits: Humans Language: Chinese Journal: Journal of Zhejiang University. Medical sciences Year: 2008 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Plasmids / Recombinant Fusion Proteins / Brain Neoplasms / RNA, Messenger / Tumor Cells, Cultured / Transfection / Glioblastoma / Cell Proliferation / Epidermal Growth Factor Limits: Humans Language: Chinese Journal: Journal of Zhejiang University. Medical sciences Year: 2008 Type: Article