Novel flutamide regulated genes in the rat ventral prostate: differential modulation of their expression by castration and flutamide treatments / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 801-808, 2007.
Article
in English
| WPRIM
| ID: wpr-310449
ABSTRACT
<p><b>AIM</b>To identify flutamide regulated genes in the rat ventral prostate.</p><p><b>METHODS</b>Total RNA from ventral prostates of control and flutamide treated rats were isolated. Differentially expressed transcripts were identified using differential display reverse transcriptase polymerase chain reaction. The effect of castration on the expression of flutamide-regulated transcripts was studied.</p><p><b>RESULTS</b>We have identified beta2-microglobulin, cytoplasmic FMR1 interacting protein 2 and pumilio 1 as flutamide induced and spermine binding protein and ribophorin II as flutamide repressed targets in the rat ventral prostate. Although flutamide treatment caused an induction of pumilio 1 mRNA, castration had no effect.</p><p><b>CONCLUSION</b>Castration and flutamide treatments exert differential effects on gene expression. Flutamide might also have direct AR independent effects, which might have implications in the emergence of androgen independent prostate cancer and the failure of flutamide therapy.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Prostate
/
Orchiectomy
/
Gene Expression Regulation
/
Beta 2-Microglobulin
/
RNA-Binding Proteins
/
Rats, Wistar
/
Intracellular Signaling Peptides and Proteins
/
Adaptor Proteins, Signal Transducing
/
Flutamide
Limits:
Animals
Language:
English
Journal:
Asian Journal of Andrology
Year:
2007
Type:
Article
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