Frequency of Y chromosome microdeletions and chromosomal abnormalities in infertile Thai men with oligozoospermia and azoospermia / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 68-75, 2007.
Article
in English
| WPRIM
| ID: wpr-310530
ABSTRACT
<p><b>AIM</b>To investigate the possible causes of oligozoospermia and azoospermia in infertile Thai men, and to find the frequencies of Y chromosome microdeletions and cytogenetic abnormalities in this group.</p><p><b>METHODS</b>From June 2003 to November 2005, 50 azoospermic and 80 oligozoospermic men were enrolled in the study. A detailed history was taken for each man, followed by general and genital examinations. Y chromosome microdeletions were detected by multiplex polymerase chain reaction (PCR) using 11 gene-specific primers that covered all three regions of the azoospermic factor (AZFa, AZFb and AZFc). Fifty men with normal semen analysis were also studied. Karyotyping was done with the standard G- and Q-banding. Serum concentrations of follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL) and testosterone were measured by electrochemiluminescence immunoassays (ECLIA).</p><p><b>RESULTS</b>Azoospermia and oligozoospermia could be explained by previous orchitis in 22.3%, former bilateral cryptorchidism in 19.2%, abnormal karyotypes in 4.6% and Y chromosome microdeletions in 3.8% of the subjects. The most frequent deletions were in the AZFc region (50%), followed by AZFb (33%) and AZFbc (17%). No significant difference was detected in hormonal profiles of infertile men, with or without microdeletions.</p><p><b>CONCLUSION</b>The frequencies of Y chromosome microdeletions and cytogenetic abnormalities in oligozoospermic and azoospermic Thai men are comparable with similarly infertile men from other Asian and Western countries.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oligospermia
/
Prolactin
/
Sex Chromosome Aberrations
/
XYY Karyotype
/
Blood
/
Luteinizing Hormone
/
Base Sequence
/
Chromosome Mapping
/
Sequence Deletion
/
DNA Primers
Limits:
Humans
/
Male
Language:
English
Journal:
Asian Journal of Andrology
Year:
2007
Type:
Article
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