PKB/Akt regulates the aggregation of actin by Girdin in mouse fertilized eggs / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1204-1211, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-310547
ABSTRACT
The purpose of this study is to reveal the role of Girdin in regulating the aggregation of actin filaments by studying the relationship between PKB/Akt and Girdin. First we used Scansite software (http//scansite.mit.edu) to predict relevant target sites of PKB/Akt on mouse Girdin. To gain insight into the role of phosphorylation of Girdin by PKB/Akt, we assessed the location of phosphorylated Girdin in fertilized eggs by staining with anti-P-Girdin 1 417 Ab. We detected a distinct increase in the fluorescence signal of F-actin and P-Girdin 1 417 after microinjection of Akt WT and myr-Akt. The addition of myr-Akt induced phosphorylation of Girdin in mouse fertilized eggs. In addition, siRNA-mediated Akt-knockdown blocked phosphorylation of Girdin. The distribution of actin filaments was obviously scattered. These results strongly suggest that PKB/Akt could directly phosphorylate Girdin on Ser1 417 and promote its function in mouse fertilized eggs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphorylation
/
Physiology
/
Zygote
/
Actins
/
Vesicular Transport Proteins
/
RNA, Small Interfering
/
Proto-Oncogene Proteins c-akt
/
Microfilament Proteins
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2016
Type:
Article
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